De novo sequencing and protein identification software

Discover More with

Proteomics mass spectrometry data analysis that’s faster, more comprehensive, easier to share, and free to use.

The New Standard in Peptide Sequencing Software.

The new standard in peptide sequencing software.

1st Novor algorithm citations
algorithm development years
million spectra analyzed

Discover More Peptide-Spectrum Matches.

Get 10-20% more peptide-spectrum matches. See what you’re missing. Have more confidence in your results.

Get Answers Faster. is 30 to 100X faster than other search engines. This means results in minutes, not hours or days.

Search All Known Sequences.

The NCBI nr database contains all sequences publicly known in any public database. No other search engine can handle its size.

Easy to Use. Easy to Share.

No need for expert tuning of tolerances and PTM settings. Our AI means that novice users can get good results even with ‘wide open’ or default parameters. Share your results with a colleague or client simply by sharing a secure link. It’s all online, so no ‘viewer’ software is required.

Technical Specifications.


  • De novo peptide sequencing
  • Protein identification – database search for peptide-spectrum matches and resulting protein IDs
  • Summary of results – de novo, peptides and protein groups all in one


  • File formats: .RAW, .mgf, .mzML, timsTOF .d
  • Databases: NCBI nr, UniProtKB/SwissProt, any FASTA format database
  • Post translational modifications supported: 40+ built in, unlimited custom, variable, fixed
  • Fragmentation modes supported: HCD, CID, ETD, EThcD
  • Enzymes supported: 11 common enzymes, semi-specific, non-specific and auto detect mode is Free to Use.

Yes, it’s free for commercial and academic use. This software is provided by Rapid Novor – a leading provider of mass spectrometry and proteomics services – we hope you enjoy the software and contact us for services if you need help. If in the future we choose to charge for the use of, it will be for premium features or higher usage. Fundamental features will remain free.

Choose for Your Next Breakthrough

  • Use the best de novo sequencing software
  • Search the largest global database
  • Get results fast
  • Easy to use

Why Choose Us for Your Next Breakthrough.

5000+ successful sequencing projects
21+ years of proteomics innovation
430+ clients served

“I am impressed!”

“The software is very good, easy to use and fast.”
Domingos Ferreira de Mélo Neto, Federal University of Ceara

“Very useful tool to identify the peptides and proteins simply based on the MS spectra. No need to specify the enzymes used to cut the protein.”

License Novor.Cloud

License the Most Powerful Peptide Sequencing Software.

Through next generation protein sequencing, Rapid Novor enables reliable discovery and development of novel reagents, diagnostics, and therapeutics.

If you’re interested in using on your own servers or private cloud? Please contact us for licensing information.

Talk to Our Scientists.

We Have Sequenced 5000+ Antibodies and We Are Eager to Help You.

Through next generation protein sequencing, Rapid Novor enables timely and reliable discovery and development of novel reagents, diagnostics, and therapeutics. Thanks to our Next Generation Protein Sequencing and antibody discovery services, researchers have furthered thousands of projects, patented antibody therapeutics, and ran the first recombinant polyclonal antibody diagnostics

Talk to our scientists. We have sequenced over 5000 antibodies and we are eager to help you.